Bovine IgM ELISA Kit 牛IgM ELISA試劑盒說(shuō)明書(shū)

Form

Stds=0-750 ng/ml ; Sample=20 ul;   Sensitivity= 15 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Kit Contents

1. Anti-Bovine IgM coated strip plate (8 wells x 12 strips) 8081 2.   Bovine IgM Std. A (0 ng/ml), 0.250 ml 8082 3. Bovine IgM Std B (25 ng/ml),   0.250 ml 8083 4. Bovine IgM Std C (50 ng/ml), 0. 250 ml 8084 5. Bovine IgM   Std D (100 ng/ml), 0. 250 ml 8085 6. Bovine IgM Std E (250 ng/ml), 0. 250 ml   8086 7. Bovine IgM Std F (500 ng/ml), 0. 250 ml 8087 8. Bovine IgM Std G (750   ng/ml), 0. 250 ml 8088 9. Sample Diluent (20x), 10ml SD-20 10. Wash Buffer   (100X), 10 ml WB-100 11. Anti-Bovine IgM-HRP Conjugate (100x), 0.12 ml 8089   12. TMB Substrate, 12 ml 80091 13. Stop solution, 12 ml 80801 14. Instruction   Manual M-8080

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300   (0.1% v/v in standards, sample diluent and HRP-conjugates

Samples

Serum. Plasma, Culture medium and   other fluids may be adapted

Crossreactivity

Cross reactivity was tested with   the following animal sera (IgM) at dilutions 1:100: Mouse (1.58%), Rat (0%),   Hamster (0%) Guinea Pig (0%), Goat (0%), Rabbit (0%), chicken (0%), Sheep   (0%), Pig (0%), Monkey (0%), Human (0%), Fetal bovine serum, FBS (0%).

Reactivity

Cow/Bovine