小鼠抗胰島素IgM檢測(cè) Mouse Anti Insulin IgM ELISA 說明書

小鼠抗胰島素IgM檢測(cè)試劑盒

Mouse Anti-Insulin IgM ELISA Kit

cat#3710-MIM

INTENDED USE

The Mouse Anti-Insulin IgM ELISA Kit is an immunoassay

suitable for quantifying or titering IgM antibody activity specific for

insulin in serum or plasma. Other biological fluids, including

tissue culture medium, may be validated for use.

GENERAL INFORMATION

Insulin-dependent diabetes mellitus, type 1 diabetes (T1D), is an

autoimmune disease in which the insulin-producing pancreatic

islets are destroyed by T lymphocytes. Studies in non-obese

diabetic (NOD) mice, an animal model for the human disease,

have shown that they develop spontaneous proliferative T cell

response to several  cell autoantigens, including insulin. It is

hypothesized that diabetes development in NOD mice is due to

the loss of tolerance to autoantigens, and a biased activation of

Th1 cells may enhance diabetes, whereas Th2 cells inhibit the

disease. In efforts to combat the autoreactive causes of T1D in

the NOD model, depletion of B cells early in development by

genetic or serological approaches and targeted elimination of

mature B cells, as well as interventions that target intracellular B

cell signaling, have been shown to provide protection against this

T cell-mediated disease.

This Mouse Anti-Insulin ELISA allows for detection and

quantitation of even very low levels of anti-insulin IgM by

providing a high discrimination between background and true

anti-insulin signals.

PRINCIPLE OF THE TEST

The Mouse Anti-Insulin IgM ELISA kit is based on the binding of

mouse anti- insulin IgM in samples to insulin immobilized on the

microwells, and anti-Insulin IgM is detected by anti-mouse IgM

specific antibody conjugated to HRP (horseradish peroxidase)

enzyme. After a washing step, chromogenic substrate (TMB) is

added and color is developed by the enzymatic reaction of HRP

on the substrate, which is directly proportional to the amount of

anti-insulin IgM present in the sample. Stopping Solution is

added to terminate the reaction, and absorbance at 450nm is

then measured using an ELISA microwell reader. The activity of

mouse IgM antibody in samples is calculated relative to mouse

anti-insulin calibrators.

PRODUCT SPECIFICATIONS

Specificity

Purified human and bovine insulin are used to coat the

microwells; thus the assay is specific for antibodies directed to

insulin. The anti-Mouse IgM HRP conjugate reacts specifically

with mouse IgM; IgA, IgG and IgE class antibodies would not be

measured above background signals.

Assay Sensitivity

The insulin coating level, HRP conjugate concentration and Low

NSB Sample Diluent are optimized to differentiate anti- insulin

IgM from background (non-antibody) signal with mouse serum

samples diluted 1:50.

 
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